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CMF Equipment

The Central Microscopy Facility supply year round and summer investigators as well as courses with instrumentation, some owned by Â黨ÐÇ¿ÕÊÓƵ and some on loan, so please read through this guide to familiarize yourself with the Facility. Feel free to contact the Facility personnel for more information. Some of the CMF rooms are on the Â黨ÐÇ¿ÕÊÓƵ One Card access system. You will need to get CMF authorization for your card to work on those CMF doors.

Scheduling Time and Trainings:
We use an on-line system by Stratocore for scheduling time and for setting up training sessions on individual microscopy systems. See this page for instructions.

Contact Louis Kerr at Marine Biological Laboratory, Woods Hole, Ma, 02543, Phone:  (508) 289-7273, Email: lkerr@mbl.edu for more information.

Electron Microscopy

TEM JEOL JEM-200CX Microscope
Microscope Name:
TEM JEOL JEM-200CX
General Overview:
80-200KV TEM equipped with:

- Low magnification mode (+/- 60 degrees tilt and 300 degree rotation)
- Standard specimen cartridge and goniometer specimen cartridge
- AMT digital side mount and bottom mount cameras

Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Electron
Detector:
Camera
Location - Building and Room
Lillie 208
Contact person:
Louie Kerr
Hourly Rate for Use:
$35/hr
SEM Zeiss Supra 40VP Microscope
Microscope Name:
SEM Zeiss Supra 40VP
General Overview: The Zeiss Supra 40VP scanning electron microscope provides high resolution surface imaging with three-dimensional appearance. it has a field emission cathode as the electron source and several detectors (SE2, inlens SE, STEM, BSD, variable pressure SE). It is equipped with an EDS (Energy Dispersive Spectrometry) system and an EBSD (Electron Backscatter Diffraction) system.
Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Electron
Features:
  • 0.1 – 30kV accelerating voltage
  • Oxford Instruments Inca Synergy 350 Energy Dispersive X-ray (EDS) microanalysis system
  • HKL Premium electron backscatter diffraction (EBSD) system
  • Leica cryo preparation and cryo stage components
  • Zeiss Atlas 5 Array Tomography Package
Detector:
Secondary electron (SE2); inlens SE; Scanning Transmission Electron Microscopy (STEM); Backscatter Detector (BSD); variable pressure Mode SE
Temperature Control:
No
CO2 Control:
No
Humidity Control:
No
Light Tight:
Yes
Location - Building and Room
Lillie 207
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use: $35/hour for standard SEM; Additional $20/hour for cryoSEM and EDS/EBSD or Array tomography

Light Microscopy

lsm710 microscope
Microscope Name:
Zeiss LSM710
General Overview:
The Zeiss LSM 710 NLO is a multi-purpose, visible light confocal laser scanning microscope. It has three independent simultaneous spectral detection channels for reflection and fluorescence and an extra detector for transmitted light. The LSM 710 is configured on an inverted Observer Z1 microscope with a motorized stage along with a variety of objectives and a temperature-controlled stage. The system is capable of confocal imaging using 7 visible lines (405, 458, 488, 514, 561, 594 and 633 nm) as well as two photon excitation using the Coherent Chameleon Vision II laser. The non-descanned system includes a 2 channel GaAsP detector for reflected light and a 2-channel module on the transmitted path.
Commercial or Pre-commercial: